Presence of Virus-specific DNA Sequences in Murine Type C Viruses

Murine and simian C-type viruses: sequences detected in the RNA of human leukemic cells by the c-DNA probes.

The detection of viral-related RNA sequences in human leukemic, human-breast cancer, and other human cancer cells has been facilitated in recent years by molecular hybridization techniques of human ribonucleic acid to the complementary DNA (C-DNA) synthesized in-vitro by animal type cRNA tumor viruses (1-5). Our laboratory has been interested in the search for such sequences in the nucleic acid...

Effects of hydroxyurea on murine type C virus-specific DNA synthesis in newly infected cells.

Cell transformation and replication of the Rauscher pseudotype of Moloney murine sarcoma virus in mouse embryo fibroblasts were inhibited by hydroxyurea within a critical time period of 30 to 90 min postinfection. In cells infected by Rauscher leukemia virus, treatment with 1mM hydroxyurea during the critical time period resulted in the accumulation of minus-strand DNA (molecular weight, 3 x 10...

Antigenic comparison of bovine type C virus with murine and feline leukemia viruses.

176 INDUCTION OF MURINE C - TYPE VIRUSES Cells

There is now considerable evidence that spontaneous leukemias in mice are caused by C-type RNA viruses. These viruses appear to be capable of existing in the animal in an unexpressed form for many generations. In inbred mouse strains such as C58 and AKR, in which virus can be detected early in life, leukemia develops at high frequency in young animals (1, 2). In other strains such as BALB/c or ...

Presence of murine leukemia virus (MLV)-related virus gene sequences in a commercial RT-PCR reagent.

BACKGROUND The recent identification of murine leukemia virus (MLV)-related viruses in patients with chronic fatigue syndrome (CFS) has aroused much interest, not least among sufferers. However, other studies failed to detect these viruses in CFS patients. METHODS We wanted to establish a MLV-related virus real-time PCR for routine diagnostics. RESULTS Our study identified false positive ML...